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Institute of Low Temperature and Structure Research PAS & Wroclaw Research Centre EIT+ Wroclaw, Poland

 

dr hab. Artur Bednarkiewicz (Prof. of INTiBS PAS)

 

Laboratory of Biospectroscopy and Bioimaging, Wroclaw Research Centre EIT+, ul. Stabłowicka 147, 54-066 Wrocław

 

Department of Spectroscopy of Excited States Institute of Low Temperature  and Structure Research, Polish Academy of Sciences, ul. Okolna 2, 50-422 Wroclaw, Poland

 

Phone : (0048) 71 39 54 166

Email : A.Bednarkiewicz(at) int.pan.wroc.pl

Description of the Organisations and PI

Institute of Low Temperature and Structural Research, PAS is a research institute of the Mathematics, Physics, Chemistry and Earth Sciences Division of Polish Academy of Sciences. Research interests include wide research of physicochemical structure of solid state and connection to physical, chemical and spectroscopic properties, especially in low temperatures. Institute’s specialty are investigations of magnetic 5f- and 4f-electron systems, superconductors, physics of phase transitions and molecular spectroscopy. The main tasks of the Institute are: conducting scientific research (mostly basic, pre-application studies); scientific training of researchers and specialists with qualifications connected with Institute’s interests and used research methods; cooperation with universities and colleges in teaching duties; smoothing the way of applying of the obtained results; supporting of the governmental units in metrological duties; and popularization of knowledge connected with Institute’s interests. Institute Scientific Council has rights to award PhD and PhD hab. Degrees in physics and chemistry.

 

EIT+ Wrocław Research Centre is the first research and technology organization in Poland that is entirely focused on the development of innovations, new technologies, and the research reflecting the needs of the modern industry. EIT+ has the most comprehensive research infrastructure in the entire East-Central Europe (totaling more than 23,000 sq. meters of area), which has been fitted with the state-of-the-art laboratory equipment. Hence, EIT+ is able to run research projects that remain interesting for the industry as well as to perform basic tests compliant with the global development trends. By employing a team of competent scientists, research project managers, technology brokers, intellectual property specialists, and commercialization specialists, the EIT+ remains capable of answering the needs of various companies, research groups, and the society.

 

Artur Bednarkiewicz received his MSc Eng. in Physics and Biomedical Engineering from the Wroclaw University of Technology in Wroclaw, Poland, in 1993 and a PhD, habilitation and Institute professorship in Physics from the Institute of Low Temperature and Structure Research, Polish Academy of Sciences, in 1998, 2013 and 2015 respectively. After postdoctoral research in the European Commission Joint Research Centre, Italy, in December 2008 he returned to ILT&SR PAS and simultaneously established an interdisciplinary team NAOMIS with the Wroclaw Research Center EIT+ focusing on Nanoparticle Assisted Molecular Imaging and Sensing. His current interdisciplinary research focuses on optical (bio)spectroscopy, novel designs of lanthanide doped nanoparticles and quantum dot biolabels, optical and spatial light modulation engineering, as well as on pure physics, spectroscopy and chemistry of lanthanide doped materials.

Description and scientific profiles of the group

Advanced measurement devices along with auxiliary optical, spectroscopic and measurement devices enable us to undertake innovative and original research in a wide area of biospectroscopy and bioimaging. In particular, the laboratory allows for:

• designing new, unique, and personalised experiments in biospectroscopy and bioimaging domain based on a fully automated optical fluorescence microscope, programmable array microscope and other advanced laboratory equipment

• synthesis, bio-functionalization, testing, analysis, and interpretation of spectroscopic properties of Stokes and anti-Stokes lanthanide-doped luminescent nano-sized labels and quantum dots

Research infrastructure

Fully automated inverse fluorescence microscope CarlZeiss AxioObserver equipped with ▪ dry objectives 10x, 20x, 40x and immersion objectives 40x, 63x (NA=1.43), ▪ ultrasensitive 1-Megapixel cameras (EMCCD) in dual-camera configuration that enable simultaneous image recording in the same area of the sample in 2 spectral ranges, ▪ standard 5 Mpix colour camera, ▪ capacity to record fluorescence spectra from under the microscope with ultrasensitive spectrograph, ▪ capacity to work in white light, phase contrast, and Nomarski interference contrast imaging modes, ▪ full automation, with the option to integrate the use of automated microscope into custom control and measurement applications (LabView), ▪ CO2 incubator + temperature control for long-term in vitro measurements. Further, the system offers:

- capability to carry out imaging in up-conversion mode (980 nm excitation, emission in Vis range) or NIR mode (808 nm excitation, 840-1050 nm emission)

- capability to provide dynamic, spatially controlled initiation of photochemical reactions (e.g. photosensitizers' activation, photolithography, etc.) using DMD technology (Digital Micro-mirror Device, Texas Instruments) integrated with the microscope. Available LED source of 375 nm wavelength and 1024x768 array allows for optical resolutions of around 10 μm.

• Extensive selection of opto-mechanical components that enables building custom optical and measurement setups

• A set of optical-fiber semiconductor and "free-running" CW, quasi-CW and pulsed lasers (including 980 nm CW 3W, 808 nm CW 2W, and picosecond lasers up to 40 MHz at 375 and 405 nm wavelength)

• Tunable Ti-sapphire laser in 700-1000 nm and 350-500 nm spectral range, under 1064 nm and 532 nm pump (>100 mJ in 10 ns pulse)

• photomultiplier tubes (400 - 1000 nm) as photodetectors and photon counters

• Power and energy meters, digital oscilloscopes, oscilloscope cards, function generator

• Shemrock SR303i (Andor) spectrograph with ultrasensitive EM-CCD detector (Newton 1600x400 back-thinned) and a photomultiplier tube, as well as 3 gratings and customized light coupling system

• Miniature CCD spectrophotometers (OceanOptics), including HR4000+ and cooled QE65000

• A set of sources, detectors and devices for measuring ultrashort fluorescence lifetime (pico-nanoseconds) using the Time Correlated Single Photon Counting (TCSPC) technique

• Capability to develop and automate custom control applications (LabView + NI Vision) and optical/spectroscopic measurement systems

• Fully equipped chemical and spectroscopy laboratory, which are ready for the synthesis and spectroscopic characterization of nano-colloidal luminophores (lanthanide doped NaYF4, CaF2, core-shell structures, CuInS2 /ZnS/CdS etc. quantum dots, colloidal gold/silver nano-particles).

Most significant recently published papers:

· Near infrared absorbing near infrared emitting highly -sensitive luminescent nanothermometer based on Nd3+ to Yb3+ energy transfer, Lukasz Marciniak, Artur Bednarkiewicz, Mariusz Stefanski, Robert Tomala, Dariusz Hreniak and   Wiesław Stręk, Phys. Chem. Chem. Phys., 2015, Accepted Manuscript, DOI: 10.1039/C5CP03861H

· Upconverting nanoparticles: assessing the toxicity, A.Gnach,   T.Lipinski,   A.Bednarkiewicz, J.Rybka and   J.A. Capobianco, Chem. Soc. Rev., 2015, Advance Article, DOI: 10.1039/C4CS00177J

· The impact of shell host (NaYF4/CaF2) and shell deposition methods on the up-conversion enhancement in Tb3+, Yb3+ codoped colloidal α-NaYF4 core–shell nanoparticles Katarzyna Prorok, Artur Bednarkiewicz, Bartlomiej Cichy, Anna Gnach, Malgorzata Misiak, Marcin Sobczyk and   Wieslaw Strek Nanoscale, 2014, 6, 1855-1864 DOI: 10.1039/C3NR05412H

· Thulium concentration quenching in the up-converting <alpha>-Tm3+/Yb3+ NaYF4 colloidal nanocrystals M.Misiak, K.Prorok, B.Cichy, A.Bednarkiewicz, W.Strek, Optical Materials 35(5), 1124–1128 (2013)

· Lanthanide doped Up-converting Nanoparticles: merits and challenges, Anna Gnach, and Artur Bednarkiewicz, NanoToday 7(6), 532–563 (2012)

· Neodymium (III) doped fluoride nanoparticles as a non-contact optical temperature sensor D.Wawrzynczyk , A.Bednarkiewicz ,  M.Nyk ,  W.Stręk,  M.Samoć, Nanoscale  4, 6959 (2012)

· Up-conversion FRET from Er3+/Yb3+:NaYF4 nanophosphor to CdSe quantum dots A. Bednarkiewicz, M.Nyk, M.Samoc, W.Strek, J. Phys. Chem. C, 114, 17535–17541 (2010)

· Enrichment of hepatocytes in a HepaRG culture using spatially selective photodynamic treatment Bednarkiewicz Artur; Rodrigues Robim M.; Whelan Maurice P., Journal Of Biomedical Optics 15(2), 028002 (2010)

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