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“LUMINESCENT NANOPARTICLES FOR BIOMEDICINE” GROUP, INSTITUTE OF PHYSICS, UNIVERSITY of TARTU, TARTU/ESTONIA

 

 

Dr. of Sciences, Ph.D., Yury (Yurii) Orlovskiy (Orlovskii) (103)

Institute of Physics, Tartu University

Ravila 14c, 50411 Tartu, Estonia

Email: yury.orlovskiy(at)ut.ee

OUR RESEARCH

During last five years our team is working in frame of two EU projects MTT50 "Design of advanced nanostructured materials with tailored properties for novel laser and light sources" and MJD167 "Rare-earth (RE) ions doped nanoparticles (NPs) for fluorescent medical diagnostics". At the same time in the frame on agreement on collaboration in scientific research between General Physics Institute (GPI) of Russian Academy of Sciences (RAS), Moscow and UT we are working together with Laboratory of Laser Biospectroscopy of GPI (Prof. V. Loschenov) for developing of new type of RE doped NPs for laser bioimaging and photothermal therapy.

Main results of our activities during last five yeras are:

  • Experimental and theoretical study of the peculiarities of the rates of spontaneous and stimulated transitions in impurity dielectric nanoparticles.
  • Experimental and theoretical studies of nonradiative energy transfer in nanosized optical materials.
  • Measurement and investigation of many-particle fluctuation kinetics of the hopping fluorescence quenching in disordered solid solutions for the first time. Development of the energy transfer probing for determining the location of –OH acceptors in the rare-earth doped fluorescence nanoparticles.
  • Development of new methods for synthesis of water dispersable nanoparticles of rare-earth compounds with controlled morphology and narrow size distribution using “soft chemistry” approaches, including microwave and microwave-hydrothermal synthesis, sol-gel, high temperature solvolysis, etc.
  • Establishing correlations between defects nature and concentration, water content and luminescence quenching in nanoparticles of rare-earth compounds.
  • Synthesis and study of the rare-earth doped crystalline nanoparticles for near IR fluorescent imaging and laser induced hyperthermia treatment. Invention of new types of heating nanoparticles.

 

METHODS

Site-selective and kinetic fluorescence spectroscopy of crystalline solids, energy transfer probe for study of morphological and structural origins of luminescence quenching, fluorescence microscopy, Monte-Carlo simulation of energy transfer kinetics, “soft chemistry” methods of synthesis of nanoparticles and their colloids.

EQUIPMENT

  • Microwave-hydrothermal laboratory device (Berghof Speedwave-4), various conventional hydrothermal autoclaves with possibility to heat solutions up to critical temperature under constant stirring and controlled gas atmosphere.
  • Chemical labware for materials preparation using soft chemistry techniques. The equipment involves spin-coater (Cookson SCS G3P-8), lab-built dip-coater, unique self-engineered system for microfibers and microtubes pulling, automatic twin syringe pump (Harvard Apparatus Model 33) for precise addition of ultra-small amounts of solutions, rotary evaporator with vacuum pump and heating bath (Büchi Rotavapor R-124).
  • Vacuum oven (SHEL LAB 1425-2), high-temperature ovens with controlled gas atmosphere, and all necessary standard labware, glassware and chemical reagents.
  • Self-engineered setup for PL experiments, covering 250 – 1100 nm spectral range, is equipped with a modern CCD spectrograph, and permits measurements and over the temperature range of 5–600 K. Time-resolved experiments (down to sub-nanosecond range) are carried out using widely tunable pulsed OPO (Ekspla) together with an intensified CCD camera, Hamamatsu PMTs, multi-channel scalers (PicoQuant, FAST Comtec), multichannel amplitude analyzer (EG&G Ortec), NIM electronic modules, and digital oscilloscopes. Numerous DPSS and semiconductor CW laser sources are also available for steady-state PL excitation.
  • Lab-built microspectroscopy setup based on Olympus BX-41M microscope unit, combined with Andor iXon EMCCD camera for highly sensitive fluorescence imaging, and a fiber-coupled Andor EMCCD spectrometer system for confocal spectroscopy. Any laser source can be used for point or wide-field excitation.
  • Renishaw inVia micro-spectrometer allowing state-of-the-art Raman and PL characterization of materials in micro scale (incl. spatial mapping). Custom-made liquid He optical cryostat and a heating/cooling stage (Linkam Scientific Instruments) are available for microspectroscopy over the temperature range of 5–600 K.
  • Closed cycle helium cryostat Janis for low-temperature measurements of luminescence from 7K.
  • Routine spectroscopic characterization: Jobin-Yvon Fluoromax-4P spectrofluorometer, Jasco V-570 spectrophotometer.

PROJECTS

  • Project No. MTT50 of European Social Fund „Design of advanced nanostructured materials with tailored properties for novel laser and light sources2011 - 2015.
  • Project No. MJD167 of European Social Fund “Rare-earth (RE) ions doped nanoparticles (NPs) for fluorescent medical diagnostics” 2011 - 2014.

MAIN PUBLICATIONS

  1. Samsonova, E. V.; Popov, A. V.; Vanetsev, A. S.; Keevend, K.; Kaldvee, K.; Puust, L.; Baranchikov, A. E.; Ryabova, A. V.; Fedorenko, S. G.; Kiisk, V.; Sildos, I.; Kikas, J.; Steiner, R.; Loschenov, V. B.; Orlovskii, Yu. V. Fluorescence quenching mechanism for water-dispersible Nd3+:KYF4 nanoparticles synthesized by microwave-hydrothermal technique, Journal of Luminescence, 2015, DOI:10.1016/j.jlumin.2015.03.015 (In press).
  2. Orlovskii, Yu.; Vanetsev, A.; Romanishkin, I.; Ryabova, A.; Pukhov, K.; Baranchikov, A.; Samsonova, E.; Keevend, K.; Sildos, I.; Loschenov, V. Laser heating of the Y1-xDyxPO4 nanocrystals, Optical Materials Express, 2015, 5(5), 1230 – 1239.
  3. Vanetsev, A.; Samsonova, E.; Gaitko, O.; Keevend, K.; Popov, A.; Mäeorg, U.; Mändar, H.; Sildos, I.; Orlovskii, Yu. Phase composition and morphology of nanoparticles of yttrium orthophosphates synthesized by microwave-hydrothermal treatment: the influence of synthetic conditions, Journal of Alloys and Compounds, 2015, 639, 415 – 421.
  4. Samsonova, E.; Popov, A.V.; Vanetsev, A.S.; Keevend, K.; Orlovskaya, E.O.; Kiisk, V.; Lange, S.; Joost, U., Kaldvee, K.; Mäeorg, U., Glushkov, N.A.; Ryabova, A.V.; Sildos, I.; Osiko, V.V.; Steiner, R.; Loschenov, V.B.; Orlovskii, Y.V. Energy Transfer Kinetics Probe for OH- Quenchers in the YPO4:Nd3+ Nanocrystals Suitable for Imaging in the Biological Tissue Transparency Window, Physical Chemistry Chemical Physics, 2014, 16, 26806 – 26815.
  5. Popov, A.V.; Orlovskii, Yu.V.; Vanetsev, A.S.; Gaitko, O.M.; Orlovskaya, E.O.; Sildos, I. Nanosecond Fluctuation Kinetics of Luminescence Hopping Quenching Originated from the 5d1 Level in the Ce3+:YPO4·0.8H2O Nanocrystals, Journal of Luminescence, 2014, 145, 774 - 778.
  6. Orlovskii Yu.V.; Popov, A.V.; Platonov, V.V.; Fedorenko, S.G; Sildos, I.; Osipov, V.V. Fluctuation kinetics of fluorescence hopping quenching in the Nd3+:Y2O3 spherical nanoparticles, Journal of Luminescence, 2013, 139, 91–97.
  7. Pukhov, K.K.; Basiev, T.T.; Duan, Chang_Kui; and Orlovskii, Yu.V. Luminescent Properties of Doped Dielectric Nanocrystals, Optics and Spectroscopy, 2013, 114/6, 868–872.
  8. Orlovskii, Yu.V.; Basiev, T.T.; Samsonova, E.V.; Glushkov, N.A.; Eliseeva, S.V.; Alimov, O.K.; Orlovskii, A.Yu.; Klimonsky, S.O. Energy Transfer Probe for Characterization of Luminescent Photonic Crystals Morphology, Journal of Luminescence, 2011, 131/3, 449 – 452.
  9. Vanetsev, A.S.; Gaitko, O.M.; Chuvashova, I.G.; Sokolov, M.N.; Tretyakov, Yu.D. Microwave Hydrothermal Synthesis of Nanodispersed YV1– xPxO4:Eu Powders// Doklady Chemistry, 2011, 441/1, 325–329.

PATENT APPLICATIONS

  • Yury Orlovskiy, Viktor Loschenov, Alexander Vanetsev, Anastasiya Ryabova, Konstantin Pukhov, Ilmo Sildos, Alexandr Popov, Materials for use in hyperthermia treatment, Patent application number 1412935.7; University of Tartu, Application date 21 July 2014, Ref. TTU02-UK.
  • Yury Orlovskiy, Viktor Loschenov, Alexander Vanetsev, Anastasiya Ryabova, Konstantin Pukhov, Ilmo Sildos, Alexandr Popov, MATERIALS FOR USE IN GENERAL HYPERTHERMIA TREATMENT, Patent application number 1418541.7; University of Tartu, Application date 19 October 2014, Ref. TTU02-UK2.

Personnel

Staff from left to right: Dr. Alexander Vanetsev, Dr. Elena Samsonova, PhD student Kerda Keevend, Master student Kaarel Kaldvee.

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