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Institut National des Sciences Appliquées de Lyon, Lyon/France

 

Dr. Alain Geloen

CarMeN Laboratory

Institut National des Sciences Appliquées de Lyon

20, avenue Albert Einstein

F-69621 Villeurbanne, France

Email: alain.geloen(at)insa-lyon.fr

 

 

INSA Lyon Team

INSA Lyon is a leading Engineering School and Research Centre driven by humanistic values that have been the basis of its model for over 50 years. INSA Lyon is one of the largest research and innovation centres in France. With its 21 laboratories, INSA Lyon applies a multi-disciplinary scientific policy aimed at excellence in partnership with other science oriented higher education establishments of the Lyon region. INSA Lyon maintains its 3d position out of the 100 best French engineering schools in the multi-criteria chart published on Thursday March 14, 2013 by L'Usine Nouvelle magazine.

Description and scientific profiles of the group

The scientists involved in the project take part of two big collaborating laboratories: Cardiovascular, Metabolism, Diabetology and Nutrition (CarMeN) Laboratory and Nanotechnology Institute of Lyon (INL). CarMeN is a biomedical research laboratory in cardiovascular diseases, metabolism, diabetology and nutrition. CarMeN is a centre of excellence in these topics where the main research teams and clinical departments in Lyon joined together with the general goal to develop strong interactions between basic research and clinical research to strengthen for a better understanding in these pathologies as well as for the follow-up of the patients. INL carries out world-leading multidisciplinary research in the areas of micro and nanotechnologies and their applications. The pioneering research undertaken at the Institute ranges from materials and technology to devices and systems, thus enabling the emergence of dedicated technologies. The Institute is supported in its work by the NanoLyon Technology Platform. The application areas cover major economic sectors: semiconductor industry, information technologies, healthcare and wellbeing, energy and the environment.

Leading researchers

Dr. Alain Geloen, CNRS Research Director, member of CarMeN Laboratory:

He has published about 100 articles in leading peer-review journals on physiology, having h-index 26 (January 2015). He has filed 4 patents on bio-applications of nanomaterials. He is an official scientific consultant of the NIVEA company. His scientific interests cover physiological studies of energy regulation in homeotherms, development of adipose tissues as well as application of nanomaterials in cell and animal physiology. Dr. Alain Geloen coordinates the INSA Lyon team and is involved in the biological part of the project, particularly in all the tests carried out on cell cultures and animal.

Dr. Vladimir Lysenko, first class CNRS Researcher, member of INL:

He has published more than 90 articles in leading peer-review journals on nanomaterials, having h-index 19 (January 2015). He has filed 11 patents on elaboration and multi-disciplinary applications of nanomaterials. His scientific profile concerns elaboration, spectroscopic studies of physico-chemical properties and multi-disciplinary applications of the IVth group nanomaterials. As a member of the INSA Lyon team, he participates in fabrication and physico-chemical characterization of the nanomaterials.

Dr. Emmanuelle Berger first class INRA (National Institute of Agrnomic research) Researcher:

She is specialized in bioinformatic analysis and molecular biology.

Other people involved in the CARTHER proposal from INL's side are:

Ass. Prof. Tetyana Nychyporuk (elaboration and physico-chemical characterization of nanomaterials) and Ass. Prof. Olivier Marty (electronic microscopy).

From left to right: Dr. Alain Geloen, Dr. Vladimir Lysenko, Dr. Emmanuelle Berger, Ass. Prof. Olivier Marty, Ass. Prof. Tetyana Nychyporuk

Research infrastructure

INSA Lyon team possesses the following research facilities which are planned to be largely used in frames of this project via:

(i) CarMeN's research infrastructure:

cell culture room, animal facilities, anesthetic system for small animals, surgery room, biochemistry laboratory, xCellignece to measure real time cell proliferation, cytation 3 for plate reading and visualization, fluorescence microscopy.

(ii) INL's research infrastructure:

NanoLyon technological platform (1200m2 with various equipments for micro-nanofabrication, nano-bio-technologies and nano-characterization), optical spectroscopies: UV-VIZ-NIR absorption, steady-state and time resolved macro- and micro- photoluminescence, FTIR, Raman scattering, dynamic light scattering, Z-potential measurements and microscopy tools: TEM, AFMRBS, FTIR, AFM

Main research axes

The researchers from the INSA Lyon team study application of the biocompatible and stable IVth group nanoparticles as theranostic agents. For example, the photos in Figure 1 highlight incorporation of the fluorescent carbon-based nanoparticles inside the 3T3-L1 fibroblast cells. Comparison between the fluorescence micrographs and the microscope photos obtained under white light demonstrates that the fluorescing nanoparticles are strongly localized inside the cells nuclei. Penetration efficiency of the nanoparticles inside the cells is studied as function of their chemical composition, size, surface chemistry and concentration.

White light and fluorescence microscopy images of biological cells (x100 magnifications) with (right column) and without (left column) the nanoparticles.

From another side, we have experimentally shown (in-vitro) and brought to the fore new therapeutic effect of the luminescent carbon-based nanoparticles. We have observed strong inhibitory effects of the nanoparticles on the cancer human cells while their effect on the healthy human cells was relatively weak (see: Mognetti et al., Preferential killing of cancer cells using silicon carbide quantum dots, J. Nanosci. Nanotechnol., vol. 10, (2010), pp. 7971-7975). Such anti-cancerous effect was found to be significantly increased with additional application of ultra-sound excitation.

5 most significant recently published papers

[1] T. Serdiuk, S. Alekseev, V. Lysenko, V. Skryshevsky, and A. Geloen, Trypsinization-dependent cell labeling with fluorescent nanoparticles, Nanoscale Res. Lett., vol. 9, (2014), N°568.

[2] T. Serdiuk, V. Lysenko, B. Mognetti, V. A. Skryshevsky, and A. Geloen, Impact of cell division on intracellular uptake and nuclear targeting with fluorescent SiC-based nanoparticles, J. Biophot., vol. 6, (2013), pp. 291-297.

[3] Yu. Zakharko, T. Serdiuk, T. Nychyporuk, A. Geloen, M. Lemiti, and V. Lysenko, Plasmon-enhanced photoluminescence of SiC quantum dots for cell imaging applications, Plasmonics, vol. 7, (2012), pp. 725-732.

[4] T. Serdiuk, Yu. Zakharko, T. Nychyporuk, A. Geloen, M. Lemiti, and V. Lysenko, Nanostructured silicon nitride thin films for label-free multicolor luminescent cell imaging, Nanoscale, vol. 4, (2012), pp. 5860-5863.

[5] T. Serdiuk, S. Alekseev, V. Lysenko, V. A. Skryshevsky, and A. Geloen, Charge-driven selective localization of fluorescent nanoparticles in live cells, Nanotech., vol. 23, (2012), N°315101.

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